Acid Casein Peptone

For the enumeration and cultivation of heterofermentative lactic acid bacteria including Lactobacilli, Streptococci Leuconostocs and lactic acid as well as microorganisms with high requirements of thiamine in meat productsApplicationsCultivation / EnumerationLACTIC ACID BACTERIAPackagingSuspend 59,5 grams of the medium in one litre of destilled water. Add 0.1 grams of actidione (dissolved in a minimum amount of 40% ethanol) and 0.2 ml of Tween 80. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°Cfor 15 minutes.FormulaPeptone 12.5Yeast Extract 7.5D(+)Glucose 10Sodium Citrate 5Thiamine Hydrochloride 0.001Sodium Chloride 5Dipotassium Hydrogen phosphate 5Manganese Chloride 0.8Ferrous sulfate 0.04Magnesium sulfate 0.14Agar 13.5